Wing Commander D.V.K. Rao, Lt. Col. Madan Deshpande, Col. R.S. Tiwari
Armed Forces Medical College, New Delhi
10 gm of dry Agnihotra ash was dissolved in 100 ml of distilled water. The solution was filtered through filter paper and was autoclaved. 0.3 ml of this fluid was distributed on 100,6 mm Whatman No.l discs so that each contains ~3μ litre of Agnihotra extract. ABST was done on various pathogenic bacteria e.g. one strain of pseudomonas, five strains of proteus, seven strains of E coli, three strains of staphylococcus pyogenes and one strain of klebiscilla by disc diffusion method. The replica method was used to find out whether the antibacterial activity of Agnihotra if any was bactericidal or bacteriostatic.
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